DNA Polymerases
T4 UvsX DNA Recombinase, Glycerol Free, G-Biosciences
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
T4 UvsX DNA Recombinase, Lyophilized, G-Biosciences
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
G-Biosciences Hot Start High Fidelity DNA Polymerase
Hot start high fidelity DNA Polymerase is used for PCR, cloning, long and high GC content template DNA amplification and diagnostics purposes.
Bsu DNA Polymerase Large Fragment, Glycerol Free, G-Biosciences
Bsu DNA Polymerase, large fragment is from Bacillus subtilis.
New England Biolabs, Inc. OneTaq DNA Polymerase – 1000 units
The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10-20% to reactions containing OneTaq GC Reaction Buffer.
New England Biolabs, Inc. Therminator™ DNA Polymerase – 1000 units
Therminator DNA Polymerase is a 9N DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides.
New England Biolabs, Inc. Bst 3.0 DNA Polymerase – 8000 units
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5' to 3' DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5' to 3' and 3' to 5' exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
New England Biolabs, Inc. EpiMark Hot Start Taq DNA Polymerase – 500 reactions
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA. Taq DNA polymerase possesses a 5- 3 polymerase activity (1,2,3) and a 5 flap endonuclease activity (4,5). EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
New England Biolabs, Inc. OneTaq DNA Polymerase – 200 units
The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10-20% to reactions containing OneTaq GC Reaction Buffer.
New England Biolabs, Inc. Q5® High-Fidelity DNA Polymerase – 500 units
Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Agilent Technologies PFU ULTRA DNA POLYMERASE
PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA polymerase for robust, ultra-high-fidelity PCR.
Cytoskeleton, Inc. SPY555-DNA Fluorogenic DNA Stain, Bright Non-Toxic, Low Background, 1 vial
SPY555-DNA is a bright, non-toxic live cell nuclear stain that enables quick and specific DNA labeling in live and fixed cells. It features very low background and is optimized for high sensitivity, making it suitable for advanced imaging techniques like confocal and STED microscopy. Its absorbance and emission properties are similar to TMR, allowing compatibility with standard filter sets, which enhances multicolor imaging alongside other dyes.