DNA Polymerases
G-Biosciences Bsu DNA Polymerase, Large Fragment
Bsu DNA Polymerase, Large fragment is used for second strand cDNA synthesis, strand displacement DNA synthesis, random primer labeling and dA-tailing.
Boca Scientific Inc SD Polymerase, 10 U/ul, 200 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
Agilent Technologies PFUTURBO DNA POLYMERASEPFUTUR
PfuTurbo DNA Polymerase, 100U (40 rxn), Contents: PfuTurbo DNA polymerase 10X cloned Pfu buffer, Applications: High performance PCR, For high fidelity PCR and primer-extension reactions, PCR cloning and generating blunt-end amplification pr
Agilent Technologies PFUTURBO DNA POLYMERASE
PfuTurbo DNA Polymerase 5 000 U (5 x 1000U) High fidelity enzyme for PCR cloning Enhances PCR product yield and length capabilities Contains: PfuTurbo DNA polymerase 10X cloned Pfu buffer
Boca Scientific Inc SD Polymerase, 10 U/ul, 1000 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
New England Biolabs, Inc. Taq DNA Polymerase with ThermoPol Buffer – 2000 units
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 53 polymerase activity and a 5 flap endonuclease activity. It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
New England Biolabs, Inc. Q5 High-Fidelity DNA Polymerase – 100 units
Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Q5 High-Fidelity DNA Polymerase – 500 units
Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. OneTaq Hot Start DNA Polymerase – 1000 units
OneTaq Hot Start DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases combined with an aptamer-based inhibitor. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields, regardless of a template's GC content. The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols. It is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution.
New England Biolabs, Inc. Q5 Hot Start High-Fidelity DNA Polymerase – 100 units
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Bst 2.0 DNA Polymerase – 8000 units
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
New England Biolabs, Inc. Q5 Hot Start High-Fidelity DNA Polymerase – 500 units
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Ab Bioscience LLC KlenTaq1 DNA Polymerase, 100ul
KlenTaq1-Increments of 100 µl enzyme (sufficient for 2000 x 25 µl reactions up to 1 kb) KlenTaq1 is a 5'-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) leaving highly active and even more heat-stable DNA polymerase activity.